2013國際會議-Berlin, Germany－陳啟煌醫師的部落格

我慕名德國豬腳餐在名店＂Mutter Hoppe"

這是剛上桌還沒吃就照起來

街道雪景真美。

受國科會研究經費贊助,我於0320夜10:10分飛德國柏林開會2天(The 3rd International Congress on Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue and Organs (CRYO) and The international congress on ONCOLOGICAL PRESPECTIVES OF FERTILITY PRESERVATION： GYNECOLOGICAL & BREAST CANCER March 21-23, 2013), 會場在 Andel's Hotel Berlin,關於冷凍最新技術(冷凍卵子,胚胎,卵巢）及婦癌專家對生殖保存的觀點及應用(早期卵巢癌,子宮內膜癌,子宮頸癌,年輕的乳癌）,我發表為癌症生殖保存新作：

ANTIAPOPTOTIC AGENT SPHINGOSINE-1-PHOSPHATE (S1P) PROTECTS AGAINST ISCHEMIC REPERFUSION INJURY OF VITRIFIED MURINE OVARIAN GRAFTS

C. Chen, C.W. Wang, C.R. Tzeng

Center for Reproductive Medicine & Sciences, Taipei Medical University Hospital, Taiwan

Objectives: Significant follicle loss of frozen ovarian graft is unavoidable due to ischemic reperfusion injury. We aim to evaluate the protective effect of antiapoptotic agent S1P on vitrified murine ovarian tissue after transplantation. Materials and Methods: 3-week-old sexually immature female FVB mice were randomly assigned to four groups: fresh, control (without S1P), 0.5mM S1P and 2mM S1P. The ovaries were immersed in S1P solution for one hour and then cryopreserved by modified vitrification (solid surface vitrification). The frozen-thawed ovaries were autotransplanted under the dorsum muscle of the animals for 10 days. The ovaries were analyzed the apoptosis-related genes caspase-3 and c-myc after vitrification and after transplantation 10 days by real-time quantitative PCR. AMH as a biomarker for ovarian reserve and follicle counts were compared 10 days after transplantation. All data were expressed as mean  SEM and analyzed by univariate analysis of variance (ANOVA). P< 0.05 was considered statistically significant. Results: The mRNA expression of caspase-3 and c-myc of the ovarian tissue shows no significant difference between the four groups after vitrification, but significantly up-regulate after transplantation in the control group. As for the ovarian reserve, AMH levels show significantly higher in the S1P groups than in the control group but lower than the fresh group, so does the effect in the primordial follicle pool. Conclusion: S1P protects against ischemic reperfusion injury of vitrified ovarian grafts rather than vitrification processes.

下機開會,會完回程,未有絲毫觀光行程,此乃第三度赴德,0325(週一)午1300抵國門,火速回生殖中心1500起看特約診及夜診,期間我以Wi-Fi follow留言並回覆,所以零距離,不孕醫師不可能完美,只有不斷進修學習,出國求新,才不負各位的努力...

我在阿姆斯特丹轉機一連上wifi就看到E大核彈級震撼催淚好孕文，看的我熱淚盈眶，一對夫妻跟了我四年取卵超過十次，一半取不到卵或沒合格胚胎植入，四年來牽掛何止是心疼與不捨，新竹台北的里程，又何止萬里，E大的中西醫，民俗療法及物理治療運動及健康食品簡直是不孕教課書努力的精典，夫妻看我之後沒外遇看別的ivf醫師，我一直感動有責任有義務，不斷進修，深夜苦讀加出國研新知，就是希望下一年比今年更好，只要各位給我機會，我仍會不斷努力的，恭喜E大夫妻獲雙寶！